Improved separation of rabbit liver metallothioneins by FPLC-ICP-MS: a comparison with the conventional anion-exchange chromatography
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منابع مشابه
Purification of DNA by anion-exchange chromatography.
Column chromatography has evolved to provide a rapid and effective alternative to more laborious methods for preparing high-quality DNA, such as CsCl-gradient centrifugation. This unit describes the use of a column made of a unique anion-exchange resin that selectively binds nucleic acids, allowing rapid separation of DNA from contaminating RNA, proteins, carbohydrates, and metabolites. The pro...
متن کاملAnion-Exchange Chromatography of Phosphopeptides: Weak Anion Exchange versus Strong Anion Exchange and Anion-Exchange Chromatography versus Electrostatic Repulsion–Hydrophilic Interaction Chromatography
Most phosphoproteomics experiments rely on prefractionation of tryptic digests before online liquid chromatography-mass spectrometry. This study compares the potential and limitations of electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) and anion-exchange chromatography (AEX). At a pH higher than 5, phosphopeptides have two negative charges per residue and are well-retained...
متن کاملDevelopments in ICP-MS: electrochemically modulated liquid chromatography for the clean-up of ICP-MS blanks and reduction of matrix effects by flow injection ICP-MS
v Chapter 1. General Introduction 1 Historical Perspectives 1 Fundamental Aspects of ICP-MS 2 ICP-MS Instrumentation 3 Blank Purity in ICP-MS 4 Previous EMLC Experiments 6 Matrix Effects in ICP-MS 7 Flow Injection in ICP-MS 9 Shielded Torch in ICP-MS 10 Dissertation Objectives and Organization 11 Figures 12 References 27 Chapter 2. Electrochemically-Modulated Liquid Chromatography for Cleaning ...
متن کاملSeparation of "uncharged" oligodeoxynucleotide analogs by anion-exchange chromatography at high pH.
Ion-exchange chromatography is a well-established method for the analysis and purification of phosphodiester-linked oligonucleotides (1). If elution is carried out under alkaline conditions, the secondary structure of Gand C-rich oligomers is disrupted. Furthermore, elution times become more sensitive to the G and T content of the oligomer, because G and T are deprotonated at pH 10 (2). In rece...
متن کاملSeparation of transferrin types in human plasma by anion-exchange high-performance liquid chromatography.
An anion-exchange high-performance liquid chromatographic procedure was developed for the separation of transferrin in human plasma. The procedure allowed the four molecular forms of transferrin, which differ with respect to bound iron, to be separated from each other and from other plasma proteins. Transferrin variants, including B and D types, could also be identified using anion-exchange hig...
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ژورنال
عنوان ژورنال: Analusis
سال: 2000
ISSN: 0365-4877,1286-482X
DOI: 10.1051/analusis:2000280351